When performing puncture biopsy of multiple nodules in the lung, 1~2 nodules with the largest diameter can be selected for puncture to clarify the nature of the nodules. The modalities of lung tissue biopsy include transbronchoscopic lung tissue biopsy, percutaneous lung puncture biopsy, and open thoracic surgery lung tissue biopsy, etc. The percutaneous lung puncture biopsy modality is usually chosen in clinical practice. The main causes of multiple nodules in the lungs include lung inflammation, lung tuberculosis, pneumoconiosis, pulmonary nodular disease, and lung tumors. In order to clarify the nature of the nodules, puncture biopsy can be performed on the nodules. For multiple nodules, it is usually sufficient to select 1~2 nodules with the largest diameter for puncture. Percutaneous puncture biopsy of multiple pulmonary nodules is to locate the lesion under the guidance of ultrasound or CT, and enter the site of the lung tissue nodule through the chest wall, and then carry out puncture and pathological examination, in order to clarify the nature of the nodule as early as possible, and to guide the clinic to carry out treatment in a timely manner.