Prostate fluid bacterial culture should pay attention to the following aspects: 1, to ensure that the standardized retention of prostate fluid: pay attention to the aseptic operation, must be operated by the clinician, through the prostate massage to retain the prostate fluid, the first drop must be removed, from the second drop of the beginning of the retention, stored in the protection of the culture medium or aseptic containers, before the end of the operation, retained 1-2 drops of prostate fluid smear, bacterial staining of the smear directly for certain Anaerobic bacterial infections can not be detected by ordinary bacterial culture, smear examination can be found suspicious; 2, correct assessment of the test results: correct assessment of the test results, because the process of prostate massage retained prostate fluid is susceptible to contamination by the skin surface of the colonizing bacteria.