Two bars on a pregnancy test paper is not necessarily pregnancy, but also related to human chorionic gonadotropin (HCG) ectopic secretion of tumors, trophoblastic tumors and so on. Pregnancy test paper is the easiest way for women to detect whether they are pregnant, mainly because the human chorionic gonadotropin in the urine of a woman after conception is significantly higher than that of a woman who is not pregnant, and the presence of two bars on the pregnancy test paper combined with the recent lack of contraception suggests, to a certain extent, the possibility of pregnancy. In addition, pregnancy test paper only indicates that HCG is elevated in a woman’s body, which may also be caused by HCG secreted by tumors such as lung, stomach, and pancreatic cancers, or by diseases such as trophoblastic tumors. Therefore, the presence of two bars on the pregnancy test paper suggests the possibility of pregnancy, but cannot be diagnosed as pregnancy, such groups can go to the hospital to complete the blood HCG test, and those who are expected to be pregnant for 5-6 weeks can further complete the vaginal ultrasound examination.