Laboratory tests of cardiac enzymology are used clinically to diagnose acute coronary syndromes. When a patient has persistent precordial pain or severe pain, in addition to observing the presence of dynamic simplicity in the electrocardiogram, it is important to check cardiac enzymes to help clarify whether the patient has suffered an acute myocardial infarction. Cardiac enzymes include creatine kinase, creatine kinase isoenzyme, aspartate aminotransferase, lactate dehydrogenase, myoglobin, troponin I or troponin T. In addition to abnormalities in cardiac enzymes when acute myocardial infarction occurs, some indicators of cardiac enzymes may also be abnormal in patients with autoimmune system diseases, acute heart failure, hypothyroidism, muscle injury, and rhabdomyolysis, for example. When a patient has anterior heart pain for more than 30 minutes, or when there is dynamic evolution of ECG, or when there is dynamic evolution of ECG and myocardial enzymes are significantly abnormal, these conditions require timely coronary angiography, as coronary angiography is the gold standard test to confirm the diagnosis of acute myocardial infarction. Cardiac enzymes have an irreplaceable role in the differential diagnosis of cardiovascular diseases, especially tests such as troponin I or troponin T. The specificity of this type of index is relatively high for the diagnosis of acute myocardial infarction, and if a patient with acute heart failure has elevated troponin I or troponin T, it often indicates a poor prognosis for the patient. Currently, it is recommended that this index be routinely tested in patients with acute heart failure. The creatine kinase isoenzyme, in addition to further clarifying whether a patient has suffered an acute myocardial infarction, can also help to assess the size of the myocardial infarction.