Hepatitis B minor tri-positive suddenly turns negative, it may be the following three cases: a. Hepatitis B minor tri-positive naturally turns negative. The rate of natural conversion of hepatitis B minor tri-positive is only 0.5% to 1.0% per year. When the surface antigen disappears for 10 years, hepatitis B virus DNA can still be detected in the liver of 14% of patients, and may still develop into hepatocellular carcinoma, but the probability is very low; second, hepatitis B minor triplets undergoing hepatitis B antiviral therapy, especially those using interferon therapy, minor triplets may turn negative; third, errors in test results, failure of test reagents, equipment failure or test specimens being The test results may suddenly turn negative. The possibility of this happening is small, but it is only necessary to go to a more professional medical institution and use a more sensitive test method for hepatitis B, namely the chemiluminescent hepatitis B quantification test, to rule out false negatives caused by the above factors.