Antinuclear antibody 1:320 is mostly positive, which may be seen in dry syndrome, systemic lupus erythematosus, rheumatoid arthritis and other diseases, and can generally be treated by medication, surgery and other means. 1. Dry syndrome: Its typical symptoms mainly include dry mouth and dry eyes. Patients can use glucocorticosteroids such as methylprednisolone, prednisone acetate; immunosuppressants such as cyclosporine, methotrexate, etc.; non-steroidal anti-inflammatory drugs such as ibuprofen, acetaminophen and other drugs for treatment as prescribed by the doctor. 2. Systemic lupus erythematosus (SLE): the main manifestations are fever, pteronyssinus erythematosus and so on. Patients can follow the doctor’s advice to use non-steroidal anti-inflammatory drugs such as celecoxib and indomethacin; anti-malarial drugs such as hydroxychloroquine and chloroquine; glucocorticosteroids such as hydrocortisone and betamethasone; and immunosuppressant drugs such as cyclophosphamide and methotrexate for treatment to relieve symptoms. 3. Rheumatoid arthritis: common symptoms are joint pain and swelling. Patients can use non-steroidal anti-inflammatory drugs such as diclofenac sodium and loxoprofen; and anti-rheumatic drugs such as leflunomide and elamectin as prescribed by the doctor. If the joint deformity occurs after unsatisfactory drug treatment, deformity correction surgery can also be adopted for treatment. Antinuclear antibody 1:320 can be caused by a variety of reasons, and it is recommended that patients go to the hospital in time to complete the relevant examinations, identify the causes, and follow the doctor’s instructions to standardize the treatment.