HPV16 positive need to consider the possibility of cervical lesions, such as cervical precancerous lesions and cervical cancer. HPV is called human papillomavirus, and HPV16 positivity indicates that the organism is infected with subtype 16 of the HPV virus, which belongs to the mucosal high-risk type, and can cause cervical pre-cancerous lesions and even cervical cancer. Cervical biopsy can be performed to check whether there are cancerous cells. If there is no lesion, recombinant human interferon treatment can be performed to improve one’s immunity and clear the infected virus. If precancerous lesions are present, surgical excision is feasible. It is necessary to maintain hygiene and cleanliness, avoid unclean sex, and conduct regular cervical checkups. Therefore, HPV16-positive patients should consult a doctor in a timely manner to clarify the specific causes of the disease, so as to avoid delaying the condition and causing irreversible damage to the body.