In some cases, after a TCT result showing ASCUS (atypical squamous cells without definite diagnosis), some doctors recommend a quantitative DNA cytology test, which is the one shown below. Nuclei? Ploidy? Each word seems to be a high level of sophistication, but what exactly does it mean? I think many people are confused, so I’ll explain it to you briefly. Cervical cell DNA ploidy quantitative analysis technology is a new “automated, standardized and intelligent” method to find tumor cells. This technique is mainly used to determine the physiological status and pathological changes of cells by measuring the DNA content or ploidy in the nucleus of specially stained cells through DNA ploidy analysis system. Normal human cells have 23 pairs of chromosomes. Under the action of carcinogenic factors, genetic mutations can occur that lead to changes in the structure or number of chromosomes and the appearance of DNA heteroploid cells. In addition, the infinite proliferation characteristic of cancer cells results in a large accumulation of DNA. By detecting the relative content of cellular DNA or the number of proliferating cells, we can determine whether the body cells have abnormalities. Simply put, if there is cervical cancer or precancerous lesion, cervical cells will have abnormal DNA ploidy, and quantitative DNA cytology can determine whether there is a possibility of cancer. The current outpatient clinics usually combine TCT and DNA quantitative analysis methods, which can improve the sensitivity and specificity of early diagnosis of cervical cancer at the same time and is more suitable for early diagnosis of cervical cancer. In particular, when “atypical squamous cells without definite diagnostic significance”, i.e. ASCUS, are present on TCT, quantitative DNA cytology can further determine the extent of the lesion and provide guidance for further triage. Studies have shown that abnormal DNA ploidy cells can be found in all levels of cervical lesions, and the higher the level of lesion, the more frequently abnormal DNA ploidy cells appear. When ASCUS was diagnosed in lesions with CIN II or higher, 71.43% of the lesions were visible or had a high number of abnormal DNA ploidy cells. Therefore, if ASCUS is diagnosed and a small number of DNA ploidy abnormal cells are not seen or are visible, most of the cervical lesions will not develop further, and such patients can be reviewed regularly as usual; however, if ASCUS shows a large number of DNA ploidy abnormal cells visible or are visible, there is a high possibility of developing cervical cancer, and in these patients, pathological biopsy is needed to further confirm the diagnosis. Finally, it should be noted that there are more and more cervical cancer screening technologies and the means of early prevention and detection of cervical cancer are very mature.