Smoking and staying up late can have an effect on routine semen tests. Sperm are mainly produced by the testes. From spermatogonia, primary spermatocytes are produced by division and eventually spermatozoa are formed. There are more factors affecting the quality of sperm, among which, smoking and staying up late are one of the common causes. After smoking, the nicotine and tar in tobacco, as well as various hazardous substances produced by tobacco combustion, may damage the sperm and lead to abnormal sperm quality. And bad lifestyle habits, such as staying up late, can also affect sperm quality. Chronic irritation over a long period of time can affect the final results of a routine semen test. Common indicators that lead to abnormal semen routine include: sperm count, sperm motility, sperm abnormality rate, and semen liquefaction time. Abnormal sperm quality may eventually lead to infertility. Therefore, in order to confirm that the patient is healthy enough to have normal offspring, it is necessary to quit bad habits and reduce the stimulation of testicular damage as well as sperm damage.