Blood should be drawn on an empty stomach at 9:00-11:00 am on day 2-4 of menstruation to measure basal sex hormone levels. Rest quietly for half an hour before the blood draw. Do not chat, play with your cell phone, do not sleep, etc. Prolactin is at its lowest level at this time and is affected by many factors such as emotional stress, sleep, hunger, stress, etc. If there is no endometrial damage, if the endometrium is less than 5 mm and there are no follicles larger than 10 mm in both ovaries, this can also be used as a base value. You can also do a progesterone test, injected intramuscularly for 3-5 days, and the value measured on the third day of blood withdrawal is also the basal value.