The Ren Vessel is the sea of yin veins, regulating yin meridian qi and blood, presiding over menstruation and raising twins; the Du Vessel is the sea of yang veins, mastering reproduction, and reflecting the functions of the brain, medulla oblongata, and kidneys. 1. Ren Vein: physiological function is to regulate yin meridian qi and blood, Ren Vein travels in the middle of the abdomen, the three yin meridians of the hand, foot, and in the abdomen and Ren Vein intersects, Ren Vein has the role of regulating yin meridian qi and blood, and Ren Vein is located in the middle of the cell, regulating the menstruation, expecting and nurturing the twins, and promoting the woman’s role in reproduction. By stimulating the acupoints of Ren Chakra, it can also treat diseases related to the lower abdomen, stomach and epigastric region, chest, throat, head and face. 2. Directing Vessel: It runs in the middle of the back, intersects with the Three Yang Meridians of the hands and feet and the Yang Wei Meridian, governs all the Yang Meridians, and is called the Sea of Yang Meridians, which is responsible for reproduction and also reflects the functions of the brain, the medulla oblongata, and the kidneys. The Directing Vessel travels upward into the brain, and stimulating the acupuncture points on the Directing Vessel can treat diseases of the mind, fever, waist and back, head and face, and so on. Both the Ren and Du Channels start in the middle of the cell and have the function of regulating the yin and yang of the whole body, and the specific operation needs to be standardized by the acupuncturist.