The third booster shot refers to the booster shot of Neocoronavirus inactivated vaccine, and it is generally recommended that nucleic acid be done at an interval of 48 hours after the third booster shot. Generally speaking, the results of nucleic acid test will not be affected after the injection of the new crown vaccine, and the third booster shot will not affect the nucleic acid results. However, the inactivated vaccine of the new crown virus is prepared from the virus through the process of cultivation and inactivation, which retains relatively intact nucleic acid fragments of the virus, even though the virus has already lost the infectiousness and pathogenicity. However, when the vaccine vial is opened, the vaccine liquid is drawn, the syringe is deflated, and the liquid residue in the syringe or the vaccine vial after vaccination may contaminate the surfaces of objects such as the vaccination table, walls, clothing of the vaccinators and vaccinees in the vaccination site. In addition, the sensitivity of nucleic acid detection is extremely high, and if sampling is performed before the nucleic acid fragments are degraded, it is likely to lead to false-positive nucleic acid test results. Therefore, it is generally recommended that nucleic acid be done 48 hours after the third booster injection. Tip: “New coronavirus pneumonia and new coronavirus pneumonia” mentioned in this article were renamed to “new coronavirus infection” on December 26, 2022, as announced by the National Health Commission.