The Autoimmune Antibody Profile 14 is one of the most important indicators for the diagnosis of connective tissue diseases, and the items included vary slightly from hospital to hospital. They include ANA, anti-dsDNA, anti-Sm antibody, anti-U1RNP antibody, anti-SSA (Ro) antibody, anti-SSB (La) antibody, anti-SCL-70 antibody, anti-Jo-1 antibody, anti-fibronectin antibody, anti-PM-SCL antibody and anti-RNA polymerase antibody, anti-nucleic dots antibody, anti-perinuclear factor antibody, anti-filament dots antibody, and so on. Autoimmune antibodies are very important indicators for the diagnosis of connective tissue diseases. For example, positive anti-Sm antibody and anti-dsDNA antibody suggest systemic lupus erythematosus; positive anti-SCL-70 antibody suggests systemic sclerosis; positive anti-Jo-1 antibody suggests myositis and dermatomyositis. Positive anti-binding point antibody suggests CREST syndrome; positive anti-U1-RNP antibody needs to be alerted to mixed connective tissue disease; positive anti-SSA and anti-SSB antibodies need to be alerted to dry syndrome or systemic lupus erythematosus. Although autoimmune antibody profile is an important diagnostic criterion, it is not the only criterion to confirm the diagnosis of the disease, but also needs to be combined with the patient’s symptoms and other tests to confirm the diagnosis. Patients are advised to go to the hospital and complete the examination to avoid delaying their condition.