The detection methods of alpha-fetoprotein include fluorescence immunoassay, enzyme immunoassay, radioimmunoassay and so on. 1. Fluorescence immunoassay: This method is to quantitatively determine the amount of alpha-fetoprotein in the patient’s serum by double antibody centrifugation. The serum samples to be measured in the alpha-fetoprotein, encapsulated recognition of the monoclonal antibody against alpha-fetoprotein, enzyme-labeled two strains of monoclonal antibodies to form a sandwich antigen-antibody reaction. Then the luminescent substrate is added, and the luminescence value is read with a microplate luminescence analyzer, and the luminescence value is proportional to the amount of alpha-fetoprotein in the serum to be tested. The luminescence value is proportional to the serum alpha-fetoprotein content to be measured. The serum alpha-fetoprotein content should be <25ng/ml under normal conditions. 2. Enzyme-linked immunoassay: this method requires the collection of patient’s serum, and then the use of alpha-fetoprotein enzyme-linked immunoassay kit for the determination of the patient’s serum, such as the results show that the positive, it means that the patient’s body alpha-fetoprotein level is high. 3. Radioimmunoassay: the use of isotope labeled and unlabeled alpha-fetoprotein, with the antibody competitive inhibition reaction to determine the concentration of alpha-fetoprotein, the current clinical application is less. There may be other ways to detect alpha-fetoprotein, consult your physician for more information.