Bile duct cancer is a relatively rare malignant tumor of the gastrointestinal tract, but its incidence has been gradually increasing in recent years. Surgical radical resection is the main treatment for cholangiocarcinoma, but the efficacy is not satisfactory. Therefore, it has become one of the hot spots in the research of bile duct cancer to understand the mechanism of the occurrence and development of bile duct cancer and to find new treatment methods to improve the efficacy of bile duct cancer. Nuclear factor kappa B (NF2κB) is a multipotent transcription factor that regulates the expression of many genes, and its expression is related to the expression of many cytokines and adhesion molecules, as well as to angiogenesis. More and more studies have shown that NF2κB is highly expressed in many tumors, including non-small cell carcinoma of the lung, lymphoma, breast cancer, colon cancer, etc., but the reports of NF2κB in cholangiocarcinoma are rare in the domestic and international literature. The overexpression of cyclinD1, a well-recognized proto-oncogene, has been shown to be associated with the exuberant division of many tumor cells. In this study, we measured the expression of NF2κB, cyclinD1 and VEGF in tumor tissues of patients with cholangiocarcinoma by immunohistochemistry, and analyzed them together with clinicopathological features to investigate the role of NF2κB in cholangiocarcinoma. Case data All cases were selected from 56 cases of bile duct cancer treated by the First Clinical School of Shanxi Medical University from 2000 to 2007, of which 31 were male and 25 were female, with a male to female ratio of 25:1; age ranged from 32 to 74 years, with an average of (55. 12 ± 4. 36) years. Among them, there were 32 cases of bile duct cancer in the hilar region, 7 cases of bile duct cancer in the middle segment, and 17 cases of bile duct cancer in the lower segment. The pathological staging was: 18 cases of highly differentiated adenocarcinoma, 26 cases of moderately differentiated adenocarcinoma, 12 cases of poorly differentiated adenocarcinoma, 25 cases with lymph node metastasis, and 18 cases with nerve infiltration. The controls were 37 cases of extrahepatic bile ducts with benign lesions (including 10 cases of congenital common bile duct cysts and 27 cases of extrahepatic bile duct stones), including 17 males and 20 females, with a male-to-female ratio of 1:1. 18; ages ranged from 26 to 68 years, with a mean of (49. 25 ± 3. 64) years. The reagents were NF2κBp65 monoclonal antibody from Santa Cruz ・248 ・ J Shanxi Med Univ 2008 Mar, 39 (3) Biotechnology, VEGF monoclonal antibody and cyclinD1 monoclonal antibody from Beijing Zhongshan Biotechnology Co. The above antibodies were purchased from Beijing Zhongshan Biotechnology Co. The primary antibody was replaced by PBS as a blank control, and known positive tissue slices were used as a positive control. The results were evaluated by the semi-quantitative scoring method proposed by Frank et al. Five different fields of view were taken under high magnification (10 × 40), and 100 cells were counted in each field. Statistical analysis All data were analyzed by SPSS13. 0 into 2 36 94. 74 Spearman’s rank correlation. 25 1 24 96.00 4.329 0. 05), see Table 2. The positive expression rate of NF2κBp65 was correlated with the degree of tissue differentiation, and the expression increased with the decrease of differentiation, and the difference was statistically significant (P < 0. 05). 27 2385 5 Statistical analysis was performed. The comparison of the immunohistochemical positivity rate, and the comparison between the histological parameters of the lower segment of the disease, such as high differentiation, medium and low differentiation38 and lymphatic metastasis, was performed by chi-square test. 10 71. 43 83. 33 Site of mass Upper segment 3284.38 0.657 >0.05 7 Middle segment nerve infiltration with 18 0 18 100.00 4.112.