It is recommended to have an ovulation 3 to 7 days prior to sperm retrieval to help maintain the quality of the sperm. Abstinence for 3~7 days is required before performing sperm test. Abstinence if too short may lead to thin semen, reduced sperm count, and so on. If the period of abstinence is too long, aging of the sperm may occur, and sperm motility and activity may be affected. There may also be dead sperm or an increase in the malformation rate of sperm, so it is recommended that sperm be emptied once 3 to 7 days before sperm retrieval. A semen test is performed by masturbation to obtain semen, and all semen is collected in a specific container and sent for testing. If the masturbation method of semen collection is difficult, a special condom can be used for collection. Once the semen specimen is collected, it needs to be kept in an environment of 20°C to 37°C. If the temperature is too high or too low, it will affect the viability of the sperm. To summarize, if there are reproductive problems, you should seek medical attention.