When doing three-generation IVF, there are condition limitations such as the existence of chromosomal abnormalities diseases, familial genetic diseases, and the occurrence of miscarriages in multiple IVF transplants, which need to be analyzed specifically. 1. Chromosomal abnormality disease: at least one of the male and female parties has chromosomal abnormality disease, in order to eugenics can be three generations of IVF. 2. Familial genetic diseases: at least one party of both sexes has familial genetic diseases, in order to prevent the transmission of genetic diseases, three generations of IVF can be carried out. 3. Miscarriage in multiple IVF transplants: If a woman has had multiple successful IVF transplants but has had a miscarriage during subsequent pregnancies, she can also undergo three-generation IVF. In addition, age over 38, recurrent miscarriages, HLA matching, etc. are also indications for IVF. If three-generation IVF is needed in the clinic, it is recommended to go to the hospital in time and make a reasonable choice under the guidance of the doctor.