Liver puncture examination has the following roles: a. For liver disease that has been clearly diagnosed, it can further understand the degree of inflammation within the liver, because sometimes liver function and inflammation within the liver cells do not correspond one to one. Clinically, it is also common to see normal liver function, but the degree of inflammation in the liver is very heavy from the puncture. In addition, we can also learn from the puncture whether there is the formation of fibrosis, or even cirrhosis, the diagnosis of cirrhosis by liver puncture will be much earlier than ultrasound and biochemistry, which is the gold standard for the diagnosis of cirrhosis. Secondly, for the hepatitis of unknown cause, some difficult and miscellaneous diseases can be diagnosed by liver puncture, such as autoimmune liver disease, hemochromatosis, glycogen accumulation disease and so on, which have special manifestations in liver pathology, and the diagnosis can be drawn by liver pathology. Third, it can determine whether the disease has improved after drug treatment, for example, after autoimmune hepatitis using hormone therapy, the degree of recovery of liver inflammation, hormone dosage can be reduced to how much, which is very helpful to clinicians, so liver puncture is a very useful examination for diagnosis of medication, efficacy judgment.