Positive autoimmune liver antinuclear antibodies may be found in healthy people, people suffering from connective tissue diseases, patients with chronic hepatitis, and patients with drug-induced liver injury. Antinuclear antibody is a general term for autoantibodies against various components of eukaryotic cells, and positive antinuclear antibody usually indicates autoimmune disorder. However, there are some healthy people who are positive for antinuclear antibodies, but the titer of antinuclear antibodies in these people is not high and they do not feel any discomfort. Positive antinuclear antibodies are most common in people with connective tissue diseases, such as autoimmune liver disease, rheumatoid arthritis, systemic lupus erythematosus, and dry syndrome. Due to the wide variety of diseases, further tests such as blood sedimentation, C-reactive protein, rheumatoid factor, and anti-mitochondrial antibodies are needed to confirm the diagnosis. Some patients with chronic hepatitis and drug-induced liver injury may also show positive antinuclear antibodies, but usually at low titers and without other manifestations of connective tissue disease.