One of the symptoms of malaria infection is that Plasmodium can be found in blood, bone marrow or sputum smears. Blood marrow or sputum smear to find Plasmodium is a method to check the disease. In the epidemic period of such disease, Plasmodium is more acute, with high fever and chills, coma and convulsions. The sudden high fever, chills and coma in infants and children in the epidemic area have a lot of impact on the health of the body and should be examined and treated in a timely manner to prevent the occurrence of other internal diseases. The principle is that Plasmodium falciparum can synthesize and secrete a stable water-soluble antigen, histidine rich protein II (HRP II), and the monoclonal antibody is prepared by dropping it on the immunochromatographic strip. The presence of histidine rich protein II in blood can be detected by the immunochromatographic strips, after adsorption, washing and color development. According to foreign reports comparing Dip-stick and other methods, the sensitivity (84.2%-93.9%) and specificity (81.1%-99.5%) of Dipstick method for malaria diagnosis are higher; it is easy to operate, fast and stable, and easy to learn. It is suitable for areas where the extent of malaria prevalence is difficult to determine, where malaria transmission is low, and where drug abuse needs to be avoided to reduce the development of resistance. It is important to note that the Dipstick method has limitations in that it is difficult to detect P. falciparum in the latent phase or with only mature gametes in the blood. 2.PCR test: At present, it is recognized that the sensitivity and specificity of PCR method is the highest among various malaria detection methods. In order to further improve the sensitivity and specificity of PCR technology as well as to facilitate the promotion in practical work, on this basis, research on nested PCR (nested PCR), PCR-ELISA and other methods has been conducted. In addition to being able to directly detect Plasmodium in anticoagulated blood samples, the technique of PCR for detecting Plasmodium on filter paper dried blood droplets has also matured, thus facilitating the monitoring of malaria in remote areas by PCR technology. Its application in the field has been limited by its high requirements for experimental techniques and conditions. In the current conditions of most malaria areas, after blood collection on site, it is still necessary to return to a laboratory with better conditions for further analysis and processing. 3.Immunological testing: ① Detection of Plasmodium antigens: Protozoa can be detected, so it can be used for clinical diagnosis of patients with current disease as well as for checking the source of infection from the population and assessing the efficacy of treatment. The main methods are agarose diffusion test, convective immunoelectrophoresis, enzyme-linked immunosorbent assay, direct fluorescence or enzyme immunostaining method, etc. ② Detection of Plasmodium antibodies: It can be used for epidemiological investigation to trace the source of infection; to infer the epidemic trend of malaria by measuring the level of antibodies in the population in the epidemic area; to over-screen blood donors to prevent malaria transfusion infection, and to evaluate the effectiveness of anti-malarial measures. In addition, for those who have had multiple episodes without identifying the cause, testing for malaria antibodies can be helpful for diagnosis. The more commonly used methods to detect antibodies include indirect fluorescent antibody test, indirect hemagglutination test, enzyme-linked immunosorbent assay, etc.