How many times of chemotherapy for cholangiocarcinoma is the best, it needs to be evaluated comprehensively with the patient’s condition, and generally 4~8 times of chemotherapy are needed. The process and frequency of chemotherapy are different for patients at different stages. Early or middle stage cancer patients are called prophylactic chemotherapy after surgery, in order to prevent recurrence and consolidate the efficacy of surgery, so the number of chemotherapy is only 4~6 times, if middle or late stage cholangiocarcinoma patients need 6~8 times of chemotherapy due to high risk of recurrence in the body, which may have dispersed tumor cells and tumor tissues. If chemotherapy shows bad effect, other treatments such as surgery or interventional embolization and arterial infusion chemotherapy can be used. Cholangiocarcinoma patients should accept treatment with positive mindset, and standardized comprehensive treatment can avoid progressive development of the disease, improve patients’ quality of life and prolong their patients’ survival time.