The United Nations Office of the High Commissioner for Human Rights (OHCHR), UN Women and the World Health Organization (WHO) have expressed the need to end the practice of virginity testing. This gynecological examination is believed to determine whether a woman or girl has had vaginal intercourse. As the world calls for the elimination of violence against women and girls around the world, this medically unnecessary, often humiliating and traumatic and painful practice must be ended. Virginity testing is a long-standing tradition, with the practice documented in at least 20 countries in all regions of the world. Women and girls are often forced to undergo virginity testing for a variety of reasons, including such requests by parents or prospective partners to determine eligibility for marriage, or by employers as a qualification for employment. Such tests are primarily administered by doctors, police or community leaders to assess the virtue, reputation or social value of women and girls. In some regions, health professionals often perform virginity testing on rape victims, allegedly to determine whether rape has in fact occurred. The general method of virginity testing is to see if the hymen is torn or the size of its opening and/or to insert a finger into the vagina for examination (the so-called “two-finger” test). Both techniques are premised on the belief that the appearance of the female genitalia can indicate whether a girl or woman has had sex. However, WHO states that there is no evidence that either method can prove whether a woman or girl has had vaginal intercourse. The term “virginity” is not a medical or scientific term. Rather, the concept of “virginity” embodies a social, cultural and religious concept that is sexist toward women and girls. The social expectation that girls and women remain “virgins” (i.e., have not had sexual intercourse) is based on stereotypes that confine female sexuality to the framework of marriage. Such beliefs are harmful to women and girls worldwide.