The six endocrine tests are of great significance for certain diseases. They include the following: First, estradiol, serum estradiol measurement is a very useful indicator for evaluating various menstrual abnormalities, such as early or delayed puberty in girls, primary or secondary amenorrhea, premature ovarian failure, etc. Second, progesterone, blood progesterone measurement is clinically used to detect ovulation and normal luteal function in non-pregnant women. Progesterone, blood progesterone measurement is used in clinic to detect ovulation and normal function of the corpus luteum in non-pregnant women, which is of special significance in determining the functional status of the corpus luteum. Testosterone, testosterone is the most important androgen in the human body, high levels of testosterone in women are generally seen in hirsutism, masculinization and so on. Fourth, prolactin, women in mid-pregnancy and this hormone with hormones, glucocorticoids and other synergistic effect on the synthesis of ovarian hormones have a certain role. Follicle-stimulating hormone, measured in the blood on the third day of menstruation, the concentration of follicle-stimulating hormone, can be used to predict the ability to conceive. Sixth, luteinizing hormone, if the luteinizing hormone is also high, then the diagnosis of ovarian failure can be determined. So the six endocrine tests are important.