Sputum testing for tuberculosis is usually better in the morning immediately after waking up, when there are fewer impurities in the sputum and the sputum volume is usually higher, which is conducive to the accuracy of the test results. Because the diagnosis of tuberculosis is relatively difficult, and the detection of positive Mycobacterium tuberculosis in sputum is the gold standard for diagnosis, the test requires the patient to try to leave a sufficient amount of sputum, and generally have to be a deep sputum in order to ensure that the test results. In the morning, sputum is relatively abundant after overnight deposition. At this time, it is more likely that the patient will be able to spit out the deep sputum, which can increase the accuracy of the test to a certain extent, and can assist the doctor to diagnose the presence of tuberculosis in the first place. The sputum test for tuberculosis generally requires the collection of sputum spit out immediately, sputum coughed up early in the morning, and sputum coughed up the night before the doctor’s visit, and three specimens need to be analyzed before the results can be accurately obtained. To summarize, it is better to cough up sputum immediately after getting up in the morning, avoid eating too much spicy and stimulating things during the sputum test for tuberculosis, and do not use drugs indiscriminately, so as not to affect the test results.