Generally the second generation of IVF can be transferred 3-5 days after egg collection, which needs to be operated by a specialized physician. The second generation of IVF is also a kind of artificial assisted reproduction technology of in vitro fertilization-embryo transfer, which refers to the single sperm follicular plasmapheresis injection technique, and is suitable for infertility caused by severe azoospermia or weak spermatozoa, and so on. Generally, after the egg is retrieved from a woman’s natural cycle or ovulation induction cycle, it needs to be cultured in vitro for 3-5 days before being transferred. After embryo transfer, it is necessary to pay attention to appropriate rest, prohibit coitus and heavy physical labor, continue to give luteal support therapy as prescribed by the doctor, and appropriately increase diet and nutrition. Generally, blood or urine pregnancy test is conducted 2 weeks after the transfer to confirm pregnancy, and ultrasonography is conducted about 4 weeks after the transfer to confirm intrauterine pregnancy, and when the embryo develops well, the obstetric examination should be conducted strictly according to the doctor’s advice. Pregnant women should pay attention to the prenatal examination during pregnancy, actively listen to the advice of professional doctors to do a comprehensive nursing care, pay attention to more rest, at the same time, should relax, avoid tension, anxiety, any discomfort should be timely to the hospital, by the doctor to clarify the diagnosis, and cooperate with the doctor to deal with the symptoms.