For samples that are positive for Mycobacterium tuberculosis by real-time fluorescent quantitative PCR, this program is performed to detect whether the patient is infected with a drug-resistant strain of Mycobacterium tuberculosis. In response to different test results, targeted treatment is carried out to achieve the goal of shortening the treatment course and increasing the cure rate. 1. Compared with the traditional method (about two weeks), this program can quickly detect (within one working day) the resistance status of Mycobacterium tuberculosis to the two first-line drugs for the treatment of tuberculosis – rifampin and isoniazid – and greatly shorten the time for the diagnosis of drug resistance to Mycobacterium tuberculosis and shorten the treatment time for patients. 2.Accurate diagnosis of drug resistance status of Mycobacterium tuberculosis can guide physicians to use drugs rationally and give full play to drug efficacy to cure TB disease in the fastest time and reduce patients’ burden. 3. Through rational use of drugs and avoidance of antibiotic abuse, the production of drug-resistant tuberculosis can be significantly reduced, and the occurrence of further drug resistance and the prevalence of multidrug-resistant tuberculosis can be prevented.