The precautions of closed antibody treatment, before the treatment should be comprehensive communication with the patient, to ensure that both husband and wife agree to carry out closed antibody treatment, before the treatment can be carried out. The patient’s past medical history should be understood, and the husband’s blood should be tested for infectious diseases, so as to rule out the absence of infectious diseases before the closed antibody treatment can be carried out. The relevant informed consent form has to be signed, informing the patient of the exact duration of immunotherapy and the number of treatments. Before the blood draw, the husband should not have fever or cold, and should not take antibiotics or other medicines within 3 days; he should stop smoking and drinking, and eat fat, sweet, greasy, spicy and stimulating food, and should have an empty stomach before the blood draw. After the injection, we should pay attention to minimize the contact with water to avoid infection, within a week of spicy and stimulating food as well as allergy-prone food, if there is a fever of more than 38 ℃, we should go to the hospital in time to see a doctor.