In outpatient clinics, we often encounter patients who come for hepatitis B markers to find out whether they are infected with hepatitis B virus. Usually, there are two clinical methods to check hepatitis B markers: qualitative and quantitative. Specifically, there are single tests for surface antigen; tests for the five indicators of hepatitis B; tests for hepatitis B nucleic acid; tests for IGM and IGG of hepatitis B core antibodies; tests for pre-S1 and DNA polymutase. Each of these tests has its own limitations and different clinical significance, so understanding them will be irreplaceable for our future application. The purpose of all the above tests is to find out whether the patient is infected with hepatitis B virus, the size of the virus replication capacity and the size of the infectiousness, the growth of antigen and antibody, and to discriminate the clinical effect of treatment. 1, surface antigen single test, qualitative and quantitative tests, the value of the high or low response to the amount of surface antigen content, and the severity of the disease is not proportional, may appear false positive or false negative, only as a screening test for the public physical examination, because of its simple and convenient and cheap and get in the grassroots application. Its positive or high value reflects the body is infected with hepatitis B virus, whether there is hepatitis also need to be combined with liver function tests to explain. 2, the five indicators of hepatitis B testing, including qualitative and quantitative testing, generally speaking, quantitative price is higher, can accurately respond to the value of various antigens and antibodies, as if the comparison of student performance has a qualitative good or bad grade and specific values to measure. It provides a deeper understanding of the infectiousness of the hepatitis B virus and the seroconversion of antigens. It is helpful in determining the treatment and prognosis. These five indicators are what we usually call the so-called major and minor triplets and what are the second and fourth positives. The positive surface antibody of hepatitis B indicates that the body has complete immunity or partial immunity to hepatitis B. 3, the test of hepatitis B nucleic acid. The price is higher, the general examination is not the first choice, more in some antiviral treatment patients regularly check, is to assess the effectiveness of treatment, whether the virus produces mutations, whether to replace the drug to provide guidance. 4, Hepatitis B core antibody IGM, IGG test; only to understand whether the recent infection of hepatitis B or the acute attack of chronic hepatitis B to do a clinical understanding. 5, before S1, DNA polymutase detection, more hepatitis B replication capacity, with the hepatitis B nucleic acid detection has the same role, the significance is not very large.