Non-invasive is not able to check for open neural tube malformations, which can be screened by choosing Down’s Screening and Systematic Fetal Structural Abnormalities Test under the guidance of a doctor. Neural tube malformation is the most common fetal birth defects, including open spina bifida, severe brain bulge, etc. During pregnancy, the initial screening can be done by Down’s screening in the middle trimester, and the fetal systematic ultrasonography at 20-24 weeks of gestation should also be performed to screen for it. The noninvasive prenatal testing technique is mainly a screening test for chromosomal abnormalities and cannot screen for open neural tube malformations. Open neural tube malformation is mainly caused by folic acid deficiency in pregnant women, so in order to prevent the occurrence of neural tube malformation. It is recommended that women who are preparing for pregnancy should start taking folic acid in the third trimester until the first three months of pregnancy. For women with high-risk factors such as a history of pregnancy with neural tube defects and a history of epilepsy, the dose of folic acid can be increased appropriately in accordance with medical advice.