Nucleic acids taken less than 24 hours apart may need to be retested, depending on local requirements. If the requirement is to perform a nucleic acid test every 24 hours, a test performed less than 24 hours apart will not fulfill the requirement. The validity of the nucleic acid test is counted from the time of sampling, so a sampling interval of less than 24 hours between tests is not considered to fulfill the requirement. If the only requirement is for a nucleic acid test to be performed within 48 hours, then the requirement is met and there is no need to perform the test again within 24 hours. Consult your local health and epidemiology department for local requirements.