Patients who wish to undergo IVF treatment for single gene genetic disorders, chromosomal abnormalities, recurrent spontaneous miscarriages, severe oligozoospermia, etc., first need to bring the genetic and chromosomal reports of the patient and the carrier to the genetic clinic, and the geneticist will collect the relevant medical history and previous testing information, and when all the information is collected completely, the geneticist will judge whether After all the information is collected, the geneticist will determine whether the patient is eligible for the three-generation IVF. Any questionable report or report from a non-authoritative institution cannot be used as a basis for disease diagnosis and will require a new genetic test at our center, which takes about 1-2 months. If the patient is deceased, blood or DNA specimens retained in an outside hospital are required, otherwise the patient cannot enter the follow-up process. When it is determined that the need for a PGD/PGS treatment is necessary, the geneticist will talk to the couple and sign a “PGD/PGS Informed Consent Form”. The signing must be done in the presence of both spouses to ensure that both parties fully understand the scope, technical means, technical limitations, success rate, cost, process and other details of the PGD/PGS treatment. After signing, both partners bring the PGD/PGS informed consent form to the female unit. Other documents to be provided include: original and copy of the couple’s ID card, original and copy of the marriage certificate, original and copy of the valid birth certificate (must be stamped with the seal of the family planning department at or above the township level and within the validity period, and their phone number). The names, birth dates and ID numbers of both spouses on the above three certificates must be the same. The following tests need to be completed before entering the IVF cycle (all tests are required to be completed at our center unless otherwise marked) Female: 1. Blood test on the 3rd day of menstruation for endocrine and syphilis, AIDS, Hepatitis B 2.5, Hepatitis C, blood type, kidney function, liver function, chromosome, AMH, TORCH 2. Physical examination (outside hospital) – breast ultrasound, electrocardiogram 3. Trichomonas, mycobacteria, mycoplasma, chlamydia, gonorrhea, cancer smear 4, luteal phase ultrasound one week before the next menstrual period (i.e. around day 21-25 of the start of this period), then register to see the female clinic and the doctor will inform whether you can enter the IVF cycle based on all the test results. Male partner: 1) Blood type, routine blood test, liver function, syphilis, HIV, hepatitis B, hepatitis C, chromosome 2) Semen analysis, mycoplasma, chlamydia, gonorrhea 3) 4 days after the test, register for the male clinic and the doctor will inform you of the precautions to take and choose according to the semen: A) Acrosome reaction or IVF pre-treatment + MAR – Tuesday or Thursday before 10 am Check before 10am B. ICSI pretreatment C. Diagnostic PTSA (non-surgical intra-testicular sperm aspiration) If you can enter the cycle, please call 63459977*0 on the 1st day of your next menstrual period to make an appointment to enter the IVF cycle, and come to our nurse station on the 2nd or 3rd day from 7:30am to 8:30am to check in, submit a copy of your three certificates and have blood drawn + negative ultrasound, and start from the same day or the next day The medication will be injected and the blood + ultrasound will be repeated every other day or every day until the egg collection, fertilization and transplantation are completed. The woman needs to stay in Shanghai for about 3 weeks for the whole procedure, please arrange for work and accommodation, we do not provide inpatient treatment. The cost of one time three generation IVF: about 60,000-70,000 RMB for single gene genetic disease and 40,000-50,000 RMB for chromosomal disease, including embryo testing fee, other examination fee, medical fee, surgery fee, etc. Since there are individual differences in the ovulation promotion process and the number of embryos varies from person to person, there may be an increase or decrease in costs due to different doses of medication and different numbers of embryos. If you have any questions during the cycle, you can consult your female doctor on the second floor after 4:00 pm. Precautions: 1. Precautions for semen collection: Abstinence for 3-5 days is required before semen examination, avoid excessive smoking or alcohol abuse, and pay attention to rest and avoid fatigue. Semen is generally collected by masturbation method, if other methods are used to collect semen should be explained to the laboratory staff. During semen collection, possible contamination should be avoided. If you have any questions, please feel free to consult with the laboratory staff or male physician. 2.After entering the cycle the male partner needs to pay attention to rest, avoid various infections, avoid contact with toxic and harmful materials, avoid overwork, avoid excessive mental tension, ensure the quality of sleep, avoid staying up late, smoking and drinking, otherwise the quality of sperm will be seriously affected. Foreign patients are advised to arrive in Shanghai one day in advance, avoid catching trains to Shanghai overnight. Ensure personal cleanliness and hygiene. The night before egg collection, the husband should take a full body bath, paying special attention to the genital area to reduce the chance of contamination of the semen. For ICSI and PTSA patients, the abstinence period can be extended to 8-10 days. Patients who cannot successfully retrieve sperm through masturbation need to visit the male department in advance. 3. Embryo testing is usually taken on the 5th day after fertilization, and the report will be issued 1-2 months after taking the material for monogenic genetic diseases and 2 weeks after taking the material for chromosomal diseases, and the transfer time will be decided by the female doctor according to the patient’s condition.