Fasting is required for transaminase test. Aminotransferase is an important indicator of liver function, and many factors, such as eating and drinking, can affect the results of aminotransferase test, so the test should be done on an empty stomach. Transaminase is an enzyme that catalyzes the transfer of amino acids between amino acids and keto acids, mainly including alanine aminotransferase and azelaic aminotransferase, and is an important indicator of liver function. When liver cells are damaged as a result of liver trauma, hepatitis, cirrhosis, liver cancer, etc., alanine aminotransferase and glutamine aminotransferase within the liver cells will be released into the bloodstream, which will increase the serum aminotransferase. Many factors can affect the results of transaminase test, such as drinking water to dilute the blood, eating greasy food to aggravate the burden on the liver, drinking alcohol, etc., which may lower or raise the transaminase and interfere with the test results. Patients should note that they should not eat or drink for 8 hours before the transaminase test, and at the same time, they should pay attention to rest, eat reasonably, and do not take drugs that damage the liver, so as not to affect the accuracy of the test results. Therefore, fasting is required for the transaminase test. When transaminase is elevated, further examination should be conducted to clarify the cause of the disease, and treatment should be carried out under the guidance of the doctor.