Gallstones formation is related to hypercholesterolemia, reduced contractility of gallbladder, long-term parenteral nutrition and other reasons. 1. Hypercholesterolemia: cholesterol, lecithin and bile salts in bile maintain the stability of bile. When hypercholesterolemia occurs, cholesterol in bile is in a supersaturated state, and it is easy to precipitate crystals and form stones. 2. Decrease in gallbladder contraction ability: bile is discharged by the contraction of the gallbladder. When the contraction ability of the gallbladder decreases, the bile in the gallbladder will be stagnant, resulting in the bile becoming viscous, which is conducive to the formation of stones. 3. Long-term parenteral nutrition: long-term parenteral nutrition patients because the gastrointestinal tract is in a state of fasting, the gallbladder usually discharges bile after eating, long-term fasting will lead to obstacles in the emptying of the gallbladder, resulting in bile siltation, concentration, and become viscous, and at the same time, there is bile pigmentation, which will gradually develop into gallstones. The causes of gallstones are very complex and related to many factors; they also include obesity, high-fat diet, long-term parenteral nutrition, diabetes mellitus, hyperlipidemia, cirrhosis of the liver, hemolytic anemia and other causes; patients who have been examined and found to have gallstones should go to the hospital in time to get a clear picture of the cause and timely treatment.