Anyone who has normal sexual life after marriage without contraception and has not conceived after two years of cohabitation is called infertile. Factors preventing conception are 60% in the woman, 30% in the man and 10% in both sexes. The effect of infertility treatment is entirely dependent on scientific, objective, comprehensive and reliable examination. 1, male examination: pay attention to whether there is a history of chronic diseases (such as tuberculosis, mumps, etc.), to understand the habits of life and whether there is any difficulty in sexual intercourse, external genital examination for deformities, semen examination for abnormalities. 2, female examination in addition to a detailed medical history, general examination, the application of the following tests: (1) gynecological examination to understand the development of internal and external genitalia, the presence of inflammation, tumors and deformities, checking the presence of breast milk. (2) Leukorrhea examination to check the pH of vagina and related inflammation and sexually transmitted diseases, including Trichomonas, Candida, Mycoplasma and Chlamydia, TORCH and other tests. (3) Tubal fluid test or hysterosalpingography. The main understanding of the tubal patency or not, as well as the uterus and fallopian tube development is normal, there is no deformity and so on. It can also be used as a treatment for tubal insufficiency (e.g. mild adhesions). The accuracy of tubal fluids is poor, and hysterosalpingography can clarify the site of obstruction. (4) Presumption of ovulation and prediction of ovulation period: it can be determined by basal body temperature measurement and cervical mucus examination or hormone measurement. (5) Hysteroscopy: to understand the situation in the uterine cavity, and to detect adhesions in the uterine cavity, submucosal fibroids, endometrial polyps, uterine deformities and so on. (6) Laparoscopy: if no abnormality is seen in the above examination, laparoscopy can be performed to understand the pelvic cavity, directly observe the uterus, fallopian tubes, ovaries with or without lesions or adhesions, and the feasible fallopian tubes through the U.S. blue liquid, under the direct visualization of the fallopian tubes to determine whether it is smooth. (7) Immunological examination: to know whether there are anti-sperm antibodies, in addition to anti-sperm antibody measurement, but also through the post-coital test, in vitro sperm penetration test and other indirect understanding. In addition, other infertility-related antibodies should also be checked.