The definitive diagnosis of psittacosis is based primarily on finding Chlamydia psittaci in blood or bronchial secretions. Psittacosis is a zoonotic disease caused by Chlamydia psittaci and is mainly transmitted from birds to humans. Most birds infected with Chlamydia psittaci have no obvious symptoms, but infection of humans can result in high fever, respiratory symptoms, and even life-threatening conditions. Diagnosis of psittacosis infection is mainly based on finding Chlamydia psittaci, which can be found by blood sampling for Chlamydia psittaci, as well as by sputum culture and bronchoscopic alveolar lavage fluid. For people with high fever, cough, and muscle aches, combined with a history of avian exposure, and when common anti-infective treatments are ineffective, it is recommended that a complete Chlamydia psittaciosis test be performed, which can help to clarify the condition.