Clostridium difficile infection can be treated with antibiotics such as vancomycin when necessary, but it should be done under the guidance of a physician, and it needs to be clarified whether it is a pathogenic strain. Clostridium difficile is an anaerobic, gram-positive bacterium, belonging to one of the normal flora of the human intestinal tract, participating in the balance of the body’s normal intestinal flora, the infected usually belong to the asymptomatic carriers, and will not cause disease; however, in the long-term application of antibiotics and other cases, Clostridium difficile may lead to opportunistic infections. For example, long-term use of amoxicillin, ceftazidime and other broad-spectrum antibiotics, resulting in intestinal flora imbalance, weakening the body’s inhibition of C. difficile, may lead to endogenous infection of C. difficile, triggering diarrhea, abdominal pain, fever and other symptoms, and in severe cases, can be fatal. For definitive pathogenic C. difficile infection, anti-infective treatment with susceptible antibiotics such as vancomycin and metronidazole may be considered, while associated broad-spectrum antibiotics usually need to be discontinued. The need for treatment of C. difficile infection and the choice of therapeutic agents should be strictly adhered to, and rationalized under ritual evaluation, and should not be used privately.