It is best to have the sex hormone 6 test on the 3rd-5th day after the menstrual period, because this period belongs to the early follicular phase, which can reflect the functional state of the ovaries and represents the patient’s basal hormone level; however, for patients who do not have a period for a long time and are eager to know the test results, the test is performed at any time, and the default time for the test is the time before the menstrual period. Since testosterone and prolactin are not restricted by the menstrual cycle, blood can be drawn on any day if only these two items are checked. It is recommended that female patients should choose the 3rd-5th day after their menstrual cycle to have their blood drawn at the hospital at 9:00 a.m. on an empty stomach for the most accurate results; for infertility or amenorrhea without menstruation for a long time, the test can be done at any time and is best done on an empty stomach; for male patients, as long as they do not have strenuous exercise and lead a regular life, they can have their blood drawn at the hospital at 9:00 a.m. on an empty stomach and sent for testing at any time.