To rule out the possibility of pregnancy after 6 days of absence of menstruation, blood HCG and ultrasound can be monitored to rule out or clarify the possibility of pregnancy by analyzing the above two results. After ruling out the possibility of pregnancy, follow-up examinations should be performed in relation to the woman’s specific age. The sex hormones should be measured, and the respective values of follicle stimulating hormone and luteinizing hormone, as well as the ratio between them, should be measured to understand the reserve function of the ovaries. The level of estradiol is measured. If estradiol is high or low, it also indicates a decrease in ovarian reserve capacity. Androgen levels are measured for hyperandrogenemia; prolactin levels are measured for hyperprolactinemia; and progesterone levels are measured for endometrial phase. The sex hormones are measured to understand the reserve function of the ovaries and to give further follow-up treatment. If the woman is after 45 years of age, it is important to consider whether she is in perimenopause, which causes a 6 day absence of menstruation.