How to test for mycoplasma in the genitourinary tract

  1, the test population from November 1, 2005 to December 23, 2008 outpatients a total of 91 cases. Among them, 68 cases were male and 23 cases were female. The subjects were taken and sent for testing strictly according to the requirements, and all patients had not used any antibiotic drugs one week before taking the specimens.  In male patients, after disinfection of the urethra, a sterile swab was used to take urethral secretions from 2-4 cm of the anterior urethra; in female patients, after disinfection of the vulva, a vaginal speculum was used to dilate the vagina, and a sterile cotton swab was used to swab away the cervical secretions, and then another sterile swab was used to reach 1-2 cm of the cervix and lightly turn around, and stay for at least 20 seconds to take The cervical secretions were sent for examination. All samples were immediately sent for testing in a matching sterile container.  3, the test method was strictly in accordance with the instructions and the results were read. uu and Mm positive results refer to the culture of the liquid at 36 ℃ for 48h from yellow to red, and the liquid is clear without turbidity. If the culture medium becomes red but cloudy, can not report positive, should consider the growth of bacteria or fungi, etc.  4, drug sensitivity determination of each antibiotic set high, low two concentrations for testing. High and low two holes do not change red said sensitive, high and low two holes are red said resistant, low hole red, high hole unchanged said intermediary.  5, statistical processing using X2 test.  Results 1, mycoplasma typing identification: 91 patients were detected in a total of 50 cases of positive mycoplasma, the positive rate of 54.95%, each type of mycoplasma infection, see Table 1, male and female infection rate by X2 test analysis, the difference is statistically significant, women significantly higher than men, see Table 2. 2, drug sensitivity results: 50 cases of mycoplasma positive patients for in vitro drug sensitivity test, each type of mycoplasma infection to The drug sensitivity results of 12 types of antibiotics are shown in Table 3, from which the resistance rate of each type of mycoplasma to various antibiotics can be found.