To check the 24-hour urine protein quantification, it is necessary to retain 24 hours of urine, need to pay attention to the time of retaining urine and the method of preservation of urine, etc.. 1. Wake up at 7:00 a.m. on the day of urine collection and empty the bladder, i.e., the first urine after waking up does not need to be collected. 2. After emptying the bladder for the first time, empty all the urine of the day into a graduated measuring cup; or empty each time into a graduated measuring cup to record the amount of urine, and then pour it into a larger container. 3. Urinate for the last time until 7:00 a.m. the next morning, which should be counted as part of the total urine output. 4. After mixing all the urine, use the urine test tube to retain 10ml of urine and send it to the hospital testing department for testing, and report the total urine volume to the testing department at the same time. At the same time, it should be noted that the urine container needs to be corrosion-resistant, washed and dried to avoid contamination; the next day at 7 o’clock even if there is no desire to urinate, but also need to try to defecate; urine retention should be kept at a low temperature as far as possible to prevent deterioration, and in summer high temperature period should be added to the preservative preservatives can be obtained in the hospital laboratory department. If the patient needs to improve the 24-hour urine protein quantification, it is recommended to go to a regular hospital to consult a professional doctor to prescribe and evaluate the results of the examination.