Second-degree contamination of amniotic fluid in babies, i.e., second-degree contamination, is usually observed for 3 to 7 days, depending on the extent of the baby’s condition. Second-degree contamination of amniotic fluid in infants indicates that the baby may have suffered from oxygen deprivation during pregnancy, causing the baby to experience increased gastrointestinal peristalsis, which results in the evacuation of meconium into the amniotic fluid, causing the amniotic fluid to be dark green in color, i.e., second-degree contamination. If the baby accidentally inhales the contaminated amniotic fluid, the baby usually needs to be observed for 5 to 7 days. However, the exact time varies according to the degree of the baby’s condition. If the baby inhaled a small amount of amniotic fluid and it did not cause an infection, it can be observed for about 3 days after treatment with oxygen. However, if the baby has inhaled a large amount of amniotic fluid and has developed meconium aspiration syndrome, the baby will need to be observed for 7 days or even longer. Parents should not be overly worried after the baby’s amniotic fluid is contaminated to the second degree, and should actively cooperate with the doctor to standardize the treatment to avoid delaying the condition.