You can determine the benign or malignant nature of the nodule by the texture of the nodule, mobility, whether the boundary of the nodule is clear, the growth rate of the nodule, and whether there is any pain when pressing on the nodule, but ultimately, only through pathological biopsy can we make clear the benign or malignant nature of the nodule. Benign anal canal nodules are mostly soft, with good mobility, clear boundaries, slow growth rate, and pain when pressed, while malignant nodules are generally hard, with poor mobility, unclear boundaries, fast growth rate, and no obvious pain when pressed. Through the above observation of the nodule, only the benign and malignant nature of the nodule can be roughly presumed, but can not confirm the diagnosis, need to take tissue from the anal nodule to do pathological biopsy, in order to clearly determine the benign and malignant nature of the nodule. Therefore, patients with anal canal nodules should go to the hospital in time, improve the examination, clarify the nature of the nodule, and take appropriate treatment in time to avoid further development of the condition.