There are many items to be checked after the 2 4D checkups, mainly including Down’s syndrome screening, gestational diabetes screening, routine physical examination and so on. 1. Down’s syndrome screening: immediately after the 4D checkup, you need to check for Down’s syndrome, the purpose is to screen the fetus for Down’s syndrome and assess the high risk of Down’s syndrome. 2. Gestational diabetes screening: generally between 24-28 weeks of pregnancy need to do a diabetes screening to understand the glucose tolerance, as well as fasting blood glucose and two-hour postprandial blood glucose values, to rule out gestational diabetes. 3. Routine physical examination: including routine blood test, urine test, weight test, height test, uterine height, abdominal circumference, etc., which is mainly based on the changes of the pregnant woman to determine the development of the embryo. The next checkup items after 2 times 4D ultrasound should be operated under the guidance of professional physicians, which can understand the development of the embryo and exclude malformation lesions in time.