To do the 24-hour urine protein quantification test for patients with kidney disease, the following three aspects are needed: First, the preparation of supplies before the test, a clean container with a capacity of 2L or more, urine specimen test tubes, preservatives, preservatives are added appropriately to prevent the deterioration of urine protein in the summer when the temperature is high. Second, women should avoid menstruation, and if the patient has a urinary tract infection, the quantitative urine protein test should be performed only after the inflammation is controlled. Third, the specific steps, starting from the early morning of each day after waking up to retain the specimen, the first urine after waking up to drain, can not be placed in the container, each subsequent urination should be placed in the container. The next morning at the same time to urinate once and put the urine into the container, measure the total amount of urine and record, then mix the urine well and take 10ml specimen for testing.