The long-term storage temperature of insulin is 2-8℃, which can be kept until the shelf life on the box, and care should be taken to avoid too much temperature fluctuation or icing during the storage process. Insulin can be stored in the refrigerator after opening for the following reasons: First, opened insulin can be stored at room temperature below 25℃ for 4 weeks without affecting the biological potency of insulin, and under normal circumstances the use time of each insulin after opening will not exceed 1 month. If the insulin in use is kept in the refrigerator, it should be taken out from the refrigerator 0.5 hours before each subcutaneous injection, and placed at room temperature before subcutaneous injection so as not to aggravate the irritation of the injection site by the low temperature. If the insulin in use is taken out from the refrigerator and injected subcutaneously directly, the low temperature will also affect the absorption of insulin and lead to excessive blood glucose fluctuations.