1. What is IVF? A: IVF is the common name for in vitro fertilization and embryo transfer (IVF-ET). This technique involves taking the eggs and sperm of an infertile couple out of the body, fertilizing them in an in vitro culture system and developing them into day 3 or 5 embryos, and then transferring the embryos into the uterine cavity in the hope of achieving a pregnancy. 2.What are the conditions to undergo IVF treatment? A: There are indications for IVF treatment: (1) female partner with gamete delivery disorder due to various factors, such as bilateral tubal obstruction; (2) ovulation disorder; (3) endometriosis, still no pregnancy after conventional drug or surgical treatment; (4) male partner with low, weak or abnormal spermatozoa, still no pregnancy after intrauterine insemination treatment, if the severity of the male partner’s factors is no longer suitable for pregnancy through If the severity of the male partner’s factors is not suitable for the implementation of intrauterine insemination techniques to obtain pregnancy. (5) Immunological infertility and unexplained infertility, who have repeatedly failed to obtain pregnancy by intrauterine insemination technique or other conventional treatments. 3.The success rate of IVF is generally about 40~50%, how is the general statistics? A: The success rate of IVF is the percentage of positive pregnancy test in the cycle of embryo transfer, for example, if there are 100 transfers and 50 positive pregnancy tests in this month, the success rate is 50%. 4.Is there any ectopic pregnancy in IVF? A: IVF technology itself cannot prevent ectopic pregnancy, nor can it reduce the risk of ectopic pregnancy. On the contrary, for those patients who may have tubal adhesions and other factors, the chance of ectopic pregnancy after IVF is slightly higher than that of the normal population. 5.What generation of IVF technology is used in the center? A: At present, there are first generation (conventional IVF technology), second generation (ICSI: single sperm intracytoplasmic microinjection technology) and IVM (immature egg in vitro maturation technology), etc. The third generation technology (PGD: pre-embryo transfer genetic diagnosis technology) is not yet available. The second generation is suitable for couples with repeated fertilization failures or serious problems with the male partner’s semen; IVM is suitable for couples with impaired egg maturation in the female partner; and the third generation is suitable for couples with genetic factors that require the selection of healthy embryos for transfer. 6.What preparation is needed for IVF treatment? A: (1) Examination of the female partner: routine physical examination and gynecological examination; hysteroscopy, laparoscopy or iodine oil imaging, immunological and other relevant examinations on the etiology of infertility; reproductive endocrine examination, examination of infectious diseases and sexually transmitted diseases. (2) Examination of the male partner: routine physical examination, routine and functional examination of semen, etiological examination, examination of infectious diseases and sexually transmitted diseases. (3) Preparation of documents: ID card of both parties, marriage certificate, original and copy of family planning service certificate of the female party, if foreign nationality or Hong Kong and Macao domicile, family planning service certificate is not required. 7.What stamp is required for the annual inspection of the family planning service certificate? On which page of the certificate? What are the specific requirements? A: The stamp of the family planning service certificate is valid within one year, if your certificate date is more than one year, you can go to the local department to stamp the “ring check” column for ring check and pregnancy check. If you are having a second child, you need to apply for a second child certificate. 8.If I have a report on the result of the chemical test, I didn’t go to get it at that time, can I go to get it again when I have a follow-up consultation? A: If it is a very important examination, the doctor needs to check the report in time, please get the report sheet and follow up the examination in time according to the doctor’s explanation; if it is a general physical examination or a routine examination before IVF, and the doctor has no special explanation, you can wait until the follow-up examination to get the report, our center will keep the paper version of the examination report for you for 3 months. 9.How can I get the test report done in the center? A: Please take the report from the nurse station on the first floor with your test receipt or invoice or outpatient medical record. 10.The male partner wants to do the examination before the IVF procedure, what are the requirements for the semen examination? Can’t have sexual intercourse before the test? A: You need to come to the center before 9:30 a.m. from Monday to Friday for the test. If you need to check semen, you should have a sperm discharge 2-5 days in advance. 11.Can the man who is a hepatitis B carrier do human insemination? Does he need to do some additional tests? A: If the male partner is a hepatitis B carrier, i.e. HbsAg(+), he can be inseminated if there is no abnormality in other items of the routine artificial insemination examination. 12.What do I need to pay attention to when I perform IVF treatment? A: You need to keep a calm mind and good sleep, pay attention to keep warm, avoid colds and fevers; eat less seafood products; avoid contact with toxic and harmful substances, such as newly decorated houses, use of cosmetics and perfumes, etc. The most important thing is to follow the medical prescriptions for medication and follow-up examinations, etc. 13.What to eat to improve the quality of embryo? A: Eat vegetables and fruits rich in vitamin C and E. If necessary, you can ask your doctor to prescribe vitamin drugs for you. 14.How many months does it take to do IVF from examination to transfer? A: For couples with normal test results and no need for special treatment, it usually takes about 1.5 months, the exact length may increase or decrease depending on the ovulation promotion program, individual’s physical condition and reaction to the drugs. 15.Is it normal to have a bloated stomach on the 8th day after using ovulation-promoting drugs? A: During the middle and late stages of ovulation medication, as the number of follicles in the ovaries increases, the volume of the follicles increases and the ovaries become larger accordingly, you may experience slight abdominal distension and pain. 16.When should I come to the center of the natural cycle? A: If your menstrual cycle is regular and you have a period every 28-30 days, you can come to the center on the 10th-12th day of your menstrual bleeding. If your menstrual cycle is irregular and you have early menstruation, it is recommended that you come to the center on the 2nd-3rd day of your menstrual bleeding (you need to check the sex hormones and ultrasound on the morning of that day) and decide the follow-up treatment according to the situation on that day. 17.Do I need to come to the center every day to monitor my follicles during my natural cycle? A: When the follicles are close to ovulation in natural cycle, you may need to come to the center for follicle monitoring and blood sampling every day. 18.The ovulation doctor said that fish and seafood are not allowed, when can I eat them? After egg retrieval or after transplantation? A: Generally, you need to be pregnant for 3 months and then consume it after the placenta is established. 19.When or how can I know the quality of embryos after egg retrieval? A: You can know the status of the transferred embryos on the day of transfer. We will inform you the status of the remaining embryos via SMS 6 days after egg retrieval. 20.How is the expected date of delivery calculated for IVF? A: Generally, 17 days before the transfer is considered as the last menstruation time, and the expected delivery date can be calculated according to this time, e.g. if the transfer took place on June 27, 2011, the last menstruation time is June 10, 2011, and the expected delivery date is March 17, 2012 (calculated from the month of the last menstruation minus 3 and the date plus 7). 21.Does it affect the fertilization of the embryo if the male partner loses his eyes and sleeps for only two hours the night before the sperm retrieval (egg retrieval day)? A: Staying up late or not getting enough sleep can affect the quality of semen. The degree of impact on embryo fertilization depends on the sperm viability after sperm retrieval. 22.What should I do if I have a semen test, but I am nervous about sperm retrieval at the hospital, which makes it difficult to retrieve sperm? A: First of all, please inform your male doctor about the problem you are facing to get help. Alternatively, you can have your sperm retrieved outside the hospital. Please record the time of the retrieval and send the specimen to the center within 30-60 minutes after the retrieval. 23.What does Elavil do? How to take it? A: Advil is a multivitamin tablet containing folic acid, zinc gluconate, etc. It helps to reduce the rate of fetal malformation, miscarriage, etc., and provides various vitamins needed for pregnant women and babies. You can start taking it from 2-3 months before pregnancy and can continue until after delivery by taking 1 tablet daily after breakfast. 24.When do both spouses need to be present at the same time during IVF treatment? A: The following situations require the presence of both spouses at the same time: 1) the day of the initial consultation; 2) the day of the medical record, which requires the writing of medical records of both men and women and the signing of an informed consent form and the provision of documents by both spouses; 3) the day of egg collection for the woman and sperm collection for the man; 4) the day of embryo transfer. 25. Can IVF be treated in hospital? A: The whole IVF treatment is carried out in outpatient clinic, and surgical operations such as egg retrieval and transfer do not require hospitalization, and you can take a short rest in the observation bed after the operation and leave the hospital on the same day. 26.If I am going to have a second transplant, should I go for a checkup during my menstrual period or after my period clears? A: If you are sure about the transplantation plan, please follow the doctor’s instructions, if not, please follow up on the 2nd-3rd day of menstrual bleeding. 27.The doctor told me on the day of transfer that all my embryos are grade 3 embryos and suggested to cancel the transfer, but I have obviously taken more than 10 eggs, why there are no embryos for transfer or freezing? A: A grade 3 embryo is an embryo in which the size of each cleavage ball is significantly different or there are more fragments in the embryo, which indicates that the embryo is of poor quality or has developmental abnormalities and the chance of getting a pregnancy by transferring them is very small. 28.Does the male partner need IVF to conceive if his semen density is very low or low? What is the general success rate of IVF? A: The male partner’s semen is affected by many factors, and the semen test results fluctuate greatly, so an accurate evaluation of the semen needs to rely on the results of at least 3 semen tests. If serious semen problems are diagnosed by the doctor, IVF treatment is needed to help conceive. The success rate of IVF is related to age, ovarian reserve function, egg quality, endometrium and immune function, etc. The doctor needs to evaluate the success rate after detailed examination. 29.The male partner has been tested several times for azoospermia, and the doctor said that the possibility of detecting sperm is very low with biopsy, so what should I do? A: If there is really no sperm after biopsy, you can consider doing artificial insemination by sperm donation or IVF according to the situation of the female partner; artificial insemination by sperm donation is treated in Guangdong Family Planning Hospital, and IVF by sperm donation is treated in our center. 30.Are there more girls born through IVF? A: The chances of delivering a boy or a girl are comparable to natural pregnancy. 31.What are the ovulation promotion drugs? A: Oral medications: Clomiphene, Letrozole, Tamoxifen, etc.; Injectable medications: Gonafen, Prilosec, Forteon, Lysine, HMG (urinary gonadotropin), Hormel, etc. 32.What is the long protocol? A: The long protocol refers to the injection of down-regulating drugs starting during the luteal phase (around day 20) of the woman’s menstruation and the use of ovulation-promoting drugs starting 14-18 days after the injection of down-regulating drugs until the follicles grow to the right size for egg retrieval. 33.What is the long injection and short injection protocol? A: Long injection protocol refers to the injection of long-acting drug, 3.75mg/stem, which needs to be injected only once per cycle; short injection protocol refers to the injection of short-acting drug, 0.1mg/stem, which needs to be injected daily until the night injection day. 34.In the descending regimen, I got menstruation before I finished the Duffylline, should I go for follow-up and hormone check early or continue to finish the injection as scheduled and go again? A: The injection of Tamiflu does not affect the menstrual cycle, so it is normal to have menstruation during the injection process. 35.Can I get some anti-inflammatory injections if I have a cold after entering the week? Will the flu affect the egg retrieval? A: If necessary, you can use anti-inflammatory drugs that are safe for pregnancy; if there are no symptoms such as fever, cough and phlegm, it usually will not affect the anesthesia for egg retrieval. 36.Can I have intercourse during this period of time as I have just started ovulation? A: Yes, during the middle and late stages of ovulation promotion, if the ultrasound shows a significant increase in ovarian tissue, the doctor will advise you to avoid strenuous exercise. 37.Is it okay for me to take the injection at night? Is it okay to do it by myself? A: If you have mastered the method of injection, you can inject at night by yourself, and it is recommended that you fix the same time of day for injection. 38.Is it necessary to inject both medicine and powder into the body? Some nurses take syringes to suck sometimes often not clean, but still leave some liquid in the bottle, the nurse said that the powder can be injected, is this correct? A: The medicine water in the promotion needle is used to dissolve the powder, generally 1-2ml is enough to dissolve the powder, the remaining solvent has no effect on the promotion, do not need to inject. 39.Do I need to wait for the results to come out before I can go up for ultrasound every time I go back for blood sampling? A: You don’t have to wait for the results of blood draw, you can see the ultrasound first, the doctor will decide whether you need to wait for the results of blood draw according to the ultrasound results. 40.When do you usually take the night shot after the ovulation? Can I take it home? How long does it take to retrieve the eggs after the night shot? A: Usually the night injection is given after 8-12 days of ovulation medication. The night injection is usually given intramuscularly or subcutaneously, and can be given at the nearest clinic or on your own; eggs are usually retrieved 34-36 hours after the night injection. 41.Does the follicle still grow after the night injection? How big is a mature follicle? A: The night injection mainly promotes the maturation of follicles. Follicles with a diameter of 14mm or more have a higher chance of maturation, while those with a diameter of 18-22mm are more mature. 42.How many days after the start of ovulation promotion to retrieve eggs? Does the male partner have to have his sperm retrieved at the time of egg retrieval? A: Usually, egg retrieval takes place about 10-14 days after the start of ovulation treatment; in general (unless the doctor gives special instructions), the male partner should retrieve the sperm on the same day as the female partner. 43. Will egg retrieval and embryo transfer be painful? A: The pain level of egg retrieval surgery is related to the location of the ovaries, the number of follicles and the individual’s physical condition. 44.How long is the operation and what should I pay attention to? A: Generally the operation takes 10-15 minutes, please note that you should abstain from drinking and eating for 8 hours before the operation. Two days before the egg retrieval, a professional nurse will give you a detailed explanation of the specific precautions to be taken and send you a note sheet. 45.What are the reasons for the lack of successful breeding after egg retrieval? Why do the staff say that some eggs cannot be cultivated when the eggs are ready to be retrieved? A: There may be no embryos after egg retrieval due to degeneration or immaturity of the eggs, or the quality of the eggs declining due to the age of the woman; the quality of the retrieved eggs can only be evaluated under a high magnification microscope in the culture room. 46. After egg retrieval, my stomach feels uncomfortable, what is wrong with it? What should I do? A: After egg retrieval, there will be a slight discomfort in the lower abdomen, which usually relieves or disappears in 2-3 days; if the number of eggs retrieved is large, there is a risk of ovarian hyperstimulation: abdominal distension, nausea, vomiting, difficulty in breathing, low urination, etc. At this time, you need to follow up as soon as possible and avoid strenuous activities. 47.What should I do if I have mild ascites and bloating? A: Drink appropriate water, eat high-protein foods, monitor the daily 24-hour total urine output, and avoid strenuous activities, and seek medical attention if necessary to help control the bloating. 48.Is embryo culture and blastocyst culture the same concept? A: Embryo culture refers to the culture of embryos up to and including day 3, while blastocyst culture generally requires culture up to and including day 5-7. 49.When is frozen embryo culture done and when can we know if the embryo is successfully cultured into blastocyst? Is the success rate of culturing fresh embryos and frozen embryos into blastocysts the same? A: Frozen embryo culture is done two days before the embryo transfer, and we can know whether the blastocyst is successfully cultured on the transfer date; the success rate of frozen embryo culture is slightly lower than that of fresh embryo. 50. How many embryos can be released in one transfer? A: According to the relevant regulations of the Ministry of Health, the maximum number of embryos that can be transferred in one cycle is 2 if the age is less than 35 years old and the first transfer is performed; the maximum number of embryos that can be transferred in the second transfer cycle or if the age is more than 35 years old (including 35 years old) is 3.