When the fifth item of the hepatitis B two-to-one test is positive, it usually indicates that the person has been infected with the hepatitis B virus but has not produced protective antibodies against hepatitis B. When this indicator is positive, it usually indicates that the person has been infected with the hepatitis B virus and the virus has been cleared through the body’s immune response after infection, but the hepatitis B surface antibody has not been produced yet. It is possible that this is a window period. Therefore, it is possible to wait for the hepatitis B surface antibody to appear on its own without the hepatitis B vaccine, or to produce the hepatitis B surface antibody through the hepatitis B vaccine. The hepatitis B vaccine should be given in three doses, according to the process of 0, 1 and 6.