Connective tissue sections are made by removing material, fixing it, dehydrating it, embedding it in wax, sectioning it, staining it, and so on. Each step of the connective tissue sectioning process is very important and complex, and each step affects the final result. The purpose of each step is to clearly show the cells in the tissue and to ensure the integrity of the cells, which is beneficial for further examination such as staining and immunohistochemistry. Connective tissue section is the most important means of clinical pathology, through the section can make the cells more clearly revealed, and in the case of staining can distinguish the cell type, guide to guide clinical diagnosis and clinical treatment. In the treatment of malignant tumors, after sectioning, immunohistochemistry and genetic testing can also be carried out, which can more accurately distinguish and classify tumors, and guide whether the corresponding targeted chemotherapy and endocrine therapy is sensitive, so as to more accurately assess the prognosis of the patient and guide the selection of treatment options. Therefore, connective tissue section has a very important clinical value.