Possible reasons for the increase of total bilirubin and glutamyl transpeptidase include physiological factors such as staying up late for a long time, and pathological factors such as hepatitis and biliary tract obstruction. 1. Physiological factors: if there are habits such as drinking alcohol, often staying up late, or excessive mental stress, it may lead to certain damage to liver cells, resulting in increased total bilirubin and glutamyl transpeptidase. 2. Pathological factors: If there are liver diseases such as viral hepatitis, gallstones, cholecystitis, drug-induced liver injury, metabolic liver disease, autoimmune liver disease, cirrhosis, liver cancer and other liver diseases, the liver cells will be seriously damaged and necrotic, which will lead to the increase of total bilirubin and glutamyl transpeptidase. It is recommended to go to a regular hospital for timely consultation, identification of the cause and timely treatment of elevated total bilirubin and glutamyl transpeptidase.