Semen is used to determine male fertility and sperm quality through a number of sperm indicators. The main indicators are as follows: i. The number of sperm, i.e. how many sperm are contained in each ml of semen. The average male is 50-75 million per milliliter, the lowest reference value is 15 million per milliliter, below 15 million per milliliter is defined as oligospermia. Second, the motility of sperm. The motility is mainly judged by the proportion of sperm swimming forward. Generally, the percentage of sperm swimming forward is above 32%, which is defined as qualified, and it is better to exceed 50. Third, the sperm deformation rate. The malformation rate and normal morphology ratio are mainly used to assess the fertilization ability of sperm. If the head of the sperm is smaller or the head is larger, or the head has more vacuoles, the tail has some curls and some cytoplasm is not shed, this type of malformed sperm often does not have the ability to fertilize. Therefore, the fifth edition of the World Health Organization’s reference standard sets the percentage of normal-form sperm at more than 4% and the malformation rate at less than 96%.